Highly Specific and Quantitative Gene Expression Profiling Based on DNA Computing

DNA chips and microarrays have been used to simultaneously analyze the expression profiling of many genes [1, 2]. In the analysis, the two-color method [3] is generally employed to determine the relative expression levels between two samples. However, not only the relative expression levels between target genes in a sample but also the absolute expression level of each target gene in a sample cannot be determined accurately with conventional DNA chips and microarrays combined with the two-color method. This hinders extensive and quantitative analysis of gene expression profiling data using bioinformatics. Here, we introduce a gene expression profiling method based on DNA computing, which enable us to know the absolute amount of target gene transcripts.